In order to prevent an epidemic of helminthic invasion, sanitary doctors in children's institutions and in food industry or public catering enterprises must necessarily wash out helminths. For their selection, washed with a 10% solution of detergent or glycerin squirrel brushes. You can also use dense cotton swabs for this purpose. In one tube, flushes are collected for helminth eggs from several homogeneous objects. Lastly, after all household items of the surveyed establishment have been processed, they must be taken from the hands of the staff. If a positive result is found, this will help to identify which of the employees violates the rules of personal hygiene and is infected with worms.
When sampling, you must follow a certain sequence. On the example of children's institutions it looks like this: the first wash for helminths, considered the main one, is taken in the food unit. Next in order are the dining room, playroom and bedroom. Lastly, the washings on the eggs of the worm should be tak
en in the washbasin and toilet. This research in children's institutions should be conducted at least once a half a year. The optimal time for taking a washout for helminths is the summer and winter periods. In medical centers, this research is conducted much more often, usually 1-3 times per quarter. A special technique is used to study the samples obtained.
Brushes or tampons with the obtained flushing sample are placed in centrifuge tubes, where they are washed in a 10% solution of glycerin or 1% sodium hydroxide. After this, the resulting liquid is passed through a centrifuge. A drop of the precipitate obtained is applied to a slide and examined under a microscope to detect the presence or absence of helminths.
In order to detect cysts of parasitic protozoa in the sample, the same procedure is used as for detecting helminth eggs, only the preparatory stage is preliminary performed. The brushes or tampons that were examined are rinsed in water, which is then poured into a 1 liter cylinder. From the flush surface, all large particles are removed, and it remains to settle for 12 hours. Then the supernatant is removed, and everything else is run through the centrifuge and viewed under a microscope.