It suffices to recall that intestinal dysbiosis is a consequence of other pathological conditions and does not have certain specific symptoms, and it can immediately be understood that in one clinical manifestation the diagnosis is simply impossible.
As the main confirmation of the imbalance in the microflora are tests for intestinal dysbiosis performed by a classical bacteriological method or more modern biochemical.
The bacteriological method provides the ability to count the number and ratio of pathological microorganisms and natural intestinal bacteria in the stool. However, regardless of the ease of conducting and accessibility of the study, the reliability of the result is affected by a large number of factors, because of which you can make quite a lot of mistakes. How to collect feces for analysis? Where to make it? How much is it done?
Assays for intestinal dysbiosis
In the analysis for dysbacteriosis, the following factors are taken into account:
- The presence of not only the cavity but also the mucosal microflora. Cavity flora are those microorganisms that are in a free state in the lumen of the intestine. Mucous flora are bacteria that are fixed on the surface of the mucous membrane. In the stool for dysbiosis, only the cavity flora is available for analysis. This fact means that its result will provide only a partial picture of which biocenoses occur in the intestine. The predominant part of the microorganisms of the inhabitants of the surface of the mucosa will not be taken into account.
- Contact of stool with air. This aspect plays a role, since it is possible that the results will be distorted even while preparing the analysis for dysbiosis. In the intestinal flora there are microorganisms-anaerobes, existing without oxygen. It turns out that when they come into contact with the air, they will begin to die, and this will not be avoided. Thus, in fact, the content of anaerobes is greater than it will be found.
- Terms of delivery of material to the laboratory, where they make tests for dysbiosis. The larger the interval between collection of feces and the delivery of the analysis, the lower the accuracy of the result, since the predominant part of the pathogenic agents will die.
To obtain an accurate result, stool tests for dysbacteriosis, starting from harvesting, should be performed according to the rules that must be observed in order to obtain a reliable result.
How to assemble the test for dysbiosis?
In practice, a large number of patients do not know how to collect feces for dysbiosis. For those who do not know how to pass the feces, the following is a list of the rules that must be observed:
- The stool to be used as a material for analysis of dysbacteriosis should be natural. It is forbidden to take various medicines to stimulate it.
- The container where the material will be stacked must be sterilized, and be sure to be tightly closed with a lid. Usually such capacity is given out in the laboratory, where the analysis for dysbiosis is made. You can use specialized containers that are sold at the pharmacy.
- The bladder is empty before the stool. Where to make a feces fence? The very act of defecation does not occur in the toilet, but in a pot, vessel or diaper. It is worth noting that all these things that are needed to collect feces, should be well washed, watered with boiling water, and the diaper is ironed.
- For the correct delivery of the analysis for dysbacteriosis, the material is taken immediately after the bowel movement, from different places 6-8 times with a spoon, attached to the container. In the event that there are any impurities in the stool, that is, blood or mucus, then they must be collected without fail. After that, the container should be closed with a tight lid.
- Collected feces are required to be handed over to the laboratory, where the analysis for dysbiosis is made no later than two hours. Cal should not be stored at room temperature. In extreme cases, you can put in a cold store, but not more than 4 hours. Freeze prepared analysis is strictly prohibited.
- Before delivering a calorie mass, there should be no medications that affect the microflora.
As you can see, the answer to the question: "How to collect feces?" Is quite simple, but the accuracy of the result will depend on compliance with the preparation of the analysis for the dysbacteriosis of these rules. How much is this training done? It will take no more than 10 minutes. Therefore, if you want accurate indicators to be obtained, you should follow these rules.
How is the analysis for dysbiosis?
First of all, the material that is required for the study is delivered at the right time at the place of analysis.
This examination for dysbacteriosis needs to be done in a bacteriological or large-scale multidisciplinary laboratory, where it is necessary to pass the feces in a specialized container.
How much analysis is done for dysbiosis? On average, the implementation takes 5-7 days.
First, sowing of stool for dysbiosis will be performed on a specialized nutrient medium, where microorganisms are given the opportunity to develop for 4 days, and sometimes more. When this time passes, the total number of colonies of microorganisms is counted, studied under a microscope and, according to a certain technique, the bacterial count per gram of material is calculated. The result will be expressed in unit CFU / g. Further results are transferred to the expert who will conduct treatment of a dysbacteriosis on the basis of the received data.
Express analysis for dysbiosis
This method, which was proposed in 2000, determines the spectrum of fatty acids. The feces are passed on to the determination of the products of the vital activity of the microorganisms of the stomach and intestine. In another way the technique can be called gas-liquid chromatographic analysis, or biochemical examination for intestinal dysbacteriosis.
The technique is more informative and simple than a bacteriological examination. In accordance with the spectrum of fatty acids, it is determined not only the disturbances in the intestinal microflora, but also which particular GI tract caused such a condition and the nature of the disease.
When compared with the classical technique, it has great advantages:
- How much rapid analysis is done for dysbiosis? Results can be obtained after an hour.
- It is possible to determine the content of the parietal microflora that remains unexplored in the case of bacteriological analysis.
- Has a high sensitivity.
- The material does not need to be handed over as soon as possible. It is possible to freeze the caloric mass and deliver it to the laboratory in this form.
To collect the feces before delivery for express diagnostics, everything should be done in the same way as when collecting material for bacteriological analysis.