Dysbacteriosis has such a symptomatology, which can be a sign of other diseases of the gastrointestinal tract. That is why effective diagnosis involves a number of specific activities.
Reliable diagnosis of dysbacteriosis is possible only in laboratory conditions, where the material was transported under certain temperature conditions of the environment, and the test is conducted no more than 3 hours after its collection. To date, not all laboratories are given the opportunity to meet all conditions, and, consequently, the results of analyzes are distorted.
Bacteriological diagnosis of intestinal dysbiosis
In the study of dysbacteriosis, an analysis of bacteriological culture will be performed first. However, such an analysis can not accurately determine the picture of the patient's condition. This can be explained by several reasons:
- Sowing can only show the state of microorganisms that are in the cavity of the large intestine, and the balance of microflora remains unknown.
- As a result, about 11-25 possible species of bacteria will be provided, although there are only 400 of them.
However, although it is not always possible to establish a diagnosis according to the rules, bacteriological examination for dysbiosis has significant advantages - relatively cheap if compared with otheranalyzes, and also with his help, the possibility of determining the type and quantity of microbes is provided.
It should be noted that reliable results of the study of the state of the intestinal microflora can give a biopsy crop, but such laboratory diagnosis of dysbiosis is very difficult to perform, along with technical problems, and therefore it is carried out only when absolutely necessary.
PCR Diagnosis of
Dysbacteriosis Over the past few years, PCR diagnostics( polymerase chain reaction) have become increasingly popular. This is due to its universal nature, since this examination for dysbacteriosis involves the taking of any material. When such an analysis of stool for the diagnosis of dysbiosis is carried out, a fragment of DNA or RNA of the pathogen will be isolated in the material, the fragment being specific only for this type of pathogen. Then the fragment must be completed to the desired size in order to visually register it.
Unlike seeding for the presence of dysbacteriosis, which will be carried out throughout the week, PCR diagnostics will give results soon after receiving the material. With its help, it is possible to determine the presence of microorganisms, even if only one bacterial cell is present, which is far from the lumen of the intestinal cavity. However, such a laboratory study of dysbacteriosis will be reliable only in the presence of known pathogens. The use of PCR is required to determine the presence of infection.
Indirect methods for the study of intestinal dysbiosis
In addition to the direct type of diagnosis, indirect use is also used. In this case, we mean the collection of stool for a laboratory study of the concentration of the products of vital activity of pathological agents, which makes it possible to determine the quantitative and qualitative composition of the intestinal microflora. Such diagnostic methods include
- Gas-liquid chromatography.
- Mass spectrometry.
The methods of breath testing are less popular, with the help of which pathogenic agents will be detected not by fecal matter sampling, but by examination of exhaled air. However, with such a study of dysbiosis, there is a high probability of errors. Such diagnostics are used only for preliminary diagnosis.
Diagnosis and treatment of dysbacteriosis
Treatment of dysbacteriosis all whole will depend on the diagnostic indicators. Thus, it is possible to identify various viruses or simple digestive tract disorders by eating poor-quality food.
In any case, you will be prescribed drugs for treatment that help restore the intestinal microflora, as well as to suppress the development of pathogenic microorganisms. Do not forget about the observance of proper nutrition, and the normalization of the entire cavity of the gastrointestinal tract. Thus, treatment of dysbacteriosis is a complex therapy, compiled only on the basis of reliable laboratory results obtained.