As a nutrient medium for the cultivation of various fungi( yeast, mold), the Saburo environment can differ in composition and depending on the components used for a variety of purposes. In the practice of growing acidophilic bacteria, yeasts and pathogenic fungi, the Saburo environment is considered to be the most nutritive medium that allows using them in laboratory analyzes and studies.
What is Saburo's environment
Being an artificial environment for feeding mushrooms, protozoa and bacteria, Saburo's environment allows to grow and count the number and to study the quality of the grown medium to control microbial contamination when using non-sterile medicines. Externally, this agent is a powder with a high degree of hygroscopicity, having a light yellow hue.
The composition of the Saburo environment can have the following:
- fish oil hydrolyzate;
- casein hydrolyzate pancreatic;
- monosodium phosphate;
- yeast extract;
- agar;
- is glucose.
There are also varieties of this type of medium, such as the Saburo peptone agar, where peptone, Saburo's medium on yeast water, Saburo's honey environment( instead of glucose or maltose, natural honey is used), Saburo's medium for storing grown crops.
To whom the study of
is assigned Since the main principle of this medium is the determination of the amount and rate of development of pathogenic microflora, when the number of fungi of different nature in the environment and the degree of development of yeasts and acidophilic bacteria are calculated on the basis of the obtained data, Saburo's medium can be used in the following cases:
- in the presence of skin lesions and, which may have a different character. With the help of the Saburo environment, it is possible to isolate the presence on the skin of strains of bacteria and yeast fungi, which are the cause of furunculosis, skin rash, inflammatory abscesses;
- in diagnosing the environment on the skin : increasing the activity of yeast fungi or acidophilus bacteria provokes frequent swelling of the tissues, hyperemia, inflammatory processes on the skin;
- for the detection of candidiasis;
- in the detection of scab, skin disease, which has a high degree of activity and is transmitted even with little contact with the infected person.
The main advantage of this procedure should be considered its effectiveness: even with a one-time exercise, it becomes possible to identify many causes of skin lesions, to establish the causes of frequently recurring inflammatory processes and abscesses.
Why do such an analysis?
Saburo's medium is prescribed if complex diagnostics is necessary in detecting many skin diseases that are caused by active reproduction of yeast fungi, acidophilus bacteria. The procedure is prescribed by a dermatologist, and the number of procedures is also calculated by him. If it is necessary to clarify the diagnosis, two or more diagnostic procedures may be prescribed for the Saburo environment.
With repeated repetition of this research procedure, there was no adverse effect on the body.
Types of procedure
Depending on the components that are used for analysis, the procedure may differ. The most commonly used are the following varieties:
- Saburo medium on yeast water;
- honey environment Saburo;
- peptone agar.
The listed species have a somewhat altered composition, but the efficacy of the procedure does not change and the reaction is used to determine the presence of an increased amount of yeast fungi and acidophilic bacteria.
On preparation of the environment Saburo will tell this video:
Indications for
- The main indications include such conditions as various skin lesions that occur excessively often and are accompanied by aggressive manifestations such as inflammatory processes, abscesses, deep furuncles.
- Changes in the skin and microflora, redness and rash also cause the appointment of this reaction for an accurate diagnosis.
- White piedra.
Contraindications for
However, with increased sensitivity of the skin to the components of the Saburo environment, this reaction is not prescribed. Other methods are used to diagnose skin diseases and to choose a scheme for their treatment.
Children's age( up to one year) and pregnancy with contraindications to such studies should also be considered contraindications to the Saburo environment.
Scheme of the Saburo environment
Is the
study safe? To a greater extent, this reaction is used to study the skin microflora in the absence of increased skin sensitivity, but in most cases it is safe for the human body.
For preparations for the Saburo environment, read below.
Preparing for the
procedure To ensure that the result of the analysis is not distorted, you should prepare for it in advance.
There are no difficulties in preparation: on the eve it is not recommended to take spicy food, do not consume alcohol, before the procedure when taking a skin sample, the skin surface is completely cleared. When taking any type of medication, tell your doctor about it.
How everything passes
Special sensations in the study of the microflora of the skin do not arise, since for the analysis, scraping from the skin is taken. Particles of the skin cover all the bacteria that are detected during this reaction.
To detect violations in the composition of microflora, a scraping is taken from the skin surface, which is placed on Saburo's medium. Further, a reaction is carried out showing the degree of occupancy of the skin by certain microorganisms( bacteria of ayidophilic origin, fungi).
Interpretation of results
For the purpose of treatment, accurate response data should be obtained, and this requires all the knowledge of the treating physician, taking into account all factors that are specific to the specific disease. For this reason, the process of deciphering the obtained analysis can be carried out only by the attending physician-dermatologist.
On the price of Saburo's nutrient medium in microbiology, let's talk about it below.
The average cost of the procedure
The price of this procedure depends on the place of its conduct, the number of repetitions of the procedure and the amount of reagents used. The cost can range from 1,280 to 1,970 rubles.
Even more useful information about the Saburo method is presented in this video: